Regulatory

Part:BBa_K1075002:Design

Designed by: Max Schelski   Group: iGEM13_Bonn   (2013-09-22)


Promoter(const.)-AraC-Term-pBAD-RBS32 (Arabinose inducable promoter system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We used the AraC-pBAD construct already characterized by iGEM Darmstadt '12 and added a medium strength RBS (RBS32) to enable direct combination with any coding region.


Source

AraC-pBAD is originally found in the genome of e. coli. We combined this part with RBS32.

References